574 Three-dimensional culture of dermal papilla cells improves their in vitro angiogenic activity

Tissue-engineered skin constructs correct vascularization remains a major challenge. We have shown that the presence of dermal papilla cells (DPCs) in these favors process, resulting better wound healing and graft take. To culture DPC as spheres increases expression angiogenic genes such VEGF, angiogenin FGF. Since angiogenesis scaffolds is essential for grafts to survive integrate with existing host tissue, our aim was compare ability monolayer sphere cultures favor endothelial cell migration neovessel development by using two study models. In assay, an increased HUVEC observed DPC-spheres conditioned media compared those monolayer. For tube formation or were seeded bottom multiwell plate covered Matrigel. Once gelled, added 72 hours. each well tubules photographed at different times. An increase total number segments, joints networks, which are characteristic hallmarks, when media. conclusion, three-dimensional DPCs improved capabilities vitro. this way, use substitutes may grafts, favoring closure